摘要 |
PROBLEM TO BE SOLVED: To create a transgenic plant resisting to infection with a different tomato spotted wilt virus (TSWV) isolate. SOLUTION: TSWV-B is characterized by cloning TSWV-B RNA, determining the sequence, and comparing the sequence with the published sequences of other TSWV isolates. The nucleotide sequences for tomato spotted wilt virus (TSWV) nucleocapsid is described, and transgenic plants each containing the nucleocapsid nucicotide sequence from a TSWV isolate is shown to provide resistance in the transgenic plant against Tospoviruses from different serogroups. In addition, transgenic plants containing the nucleocapsid nucleotide sequence from a lettuce isolate of TSWV are produced, and plants producing larger amounts of the nucleocapsid protein are found to possess moderate levels of protection against both the homologous isolate and isolates of distantly related Impatiens necrotic spot virus (INSV). COPYRIGHT: (C)2006,JPO&NCIPI
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