| 摘要 |
The invention relates to a method for the in-vitro determination, especially typification, of well or fully differentiated mammal cells comprising the steps of: a) irradiating the cells with infrared light and recording the absorption spectrum obtained; b) carrying out a Fourier transformation of the absorption spectrum from (a) to obtain an FT-IR spectrum; c) calculating the first derivative and, optionally, higher derivatives of the FT-IR spectrum; and d) comparing the derivative(s) from step (c) in a preselected wave number range with the same derivative(s) of reference FT-IR spectra determined earlier; and an apparatus for the in-vitro determination, especially typification, of well or fully differentiated mammal cells, comprising: a) a sample-receiving device for a suspension of the well or fully differentiated mammal cells; b) a means for irradiating the cells with infrared light and a means for recording the absorption spectrum obtained; c) a means for carrying out a Fourier transformation of the absorption spectrum obtained into an FT-IR spectrum; d) a means for calculating the first derivative and, optionally, further derivatives of the FT-IR spectrum; and e) a means for comparing the derivative(s) from step (d) in a preselected wave number range with the same derivative(s) of reference FT-IR spectra prepared earlier and stored in the apparatus.
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