METHOD FOR STABILISING REAGENTS WHICH ARE USEFUL FOR NUCLEIC ACID AMPLIFICATION

摘要

A method for stabilising reagents suitable for use in a nucleic acid amplification reaction has now been developed comprising: (i) preparing a reagent mixture comprising reagents suitable for use in a nucleic acid amplification reaction wherein the mixture comprises a polynucleotide polymerase; and (ii) drying the reagents; characterised in that said reagent mixture comprises from about 0.1 % to about 50 % of the final concentration of magnesium ions required to activate an amplification reaction. Reagents, reaction vessels, use of such reagents in a nucleic acid amplification reaction, and a method of conducting an amplification reaction using reagents so prepared are also disclosed herein.