DYNAMIC CHEMICAL IMAGING OF BIOLOGICAL CELLS AND OTHER SUBJECTS
摘要
The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.
申请公布号
WO2006005022(A2)
申请公布日期
2006.01.12
申请号
WO2005US23638
申请日期
2005.06.30
申请人
CHEMIMAGE CORPORATION;MAIER, JOHN, S.;TREADO, PATRICK, J.;VOIGT, THOMAS, C.;TUSCHEL, DAVID
发明人
MAIER, JOHN, S.;TREADO, PATRICK, J.;VOIGT, THOMAS, C.;TUSCHEL, DAVID