发明名称 |
THE METHOD SELECTING HIGHLY SPECIFIC PROBES FOR HPV GENOTYPE ANALYSIS AND THE PROBES THEREOF |
摘要 |
<p>A method for selecting a highly specific probe among a predetermined range of nucleotide sequences comprises: setting a group of nucleotide sequences among the predetermined range of nucleotide sequences; setting a range of nucleotide sequences in the group of nucleotide sequences; selecting first candidate probes having a certain length within the range of nucleotide sequences; selecting second candidate probes whose melting temperature with target nucleic acids for the first candidate probes is in an appropriate range; selecting third candidate probes whose melting temperature with a specific set of nucleotide sequences is lower than a hybridization temperature; and selecting fourth candidate probes, wherein a secondary structure of each fourth candidate probe has a melting temperature lower than the hybridization temperature by approximately 5° C. to approximately 10° C. and higher than a temperature that is lower than the hybridization temperature by approximately 5° C. to approximately 10° C.</p> |
申请公布号 |
KR20060003253(A) |
申请公布日期 |
2006.01.10 |
申请号 |
KR20040052068 |
申请日期 |
2004.07.05 |
申请人 |
BIOMEDLAB, CO. |
发明人 |
HWANG, CHANG IL;CHO, YONG KU |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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