摘要 |
FIELD: gene engineering, in particular new bacterium Escherichia coli strain useful in production of high purified gene engineered human insulin. ^ SUBSTANCE: bacterium Escherichia coli strain BL21/pPINS07(BL07) which represents producer of recombinant human proinsulin is obtained by transformation E.coli BL21 cells with plasmid DNA pPINS07 governing of proinsulin synthesis. ^ EFFECT: recombinant human proinsulin with increased cell stability. ^ 1 ex |