| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a culturing method for obtaining extremely more animal cells compared to a conventional method by promoting proliferation and differentiation-inducing of the cultured animal cells in the proliferation culture and differentiation-inducing culture of stem cells or the like of various kinds of animals while effectively keeping the differentiation abilities of the animal stem cells or the like under proliferation. <P>SOLUTION: The proliferation of the cultured animal cells of various kinds of the animal cells is promoted by culturing the animal cells in the presence of an extracellular substrate derived from a mesenchymal cell, an epithelial cell, a cartilaginous precursor cell or a fibroblast. Further, the differentiation ability of the cultured animal stem cells or the like is effectively maintained under the proliferation, and the differentiation induction is promoted to enable the excellent differentiation-inducing culture of the animal stem cells to be carried out. Preferably, even serum in a low concentration can be effectively proliferated and the culture by using the human serum can be carried out by using the method of culturing the animal cells and adding various kinds of additives to a medium. <P>COPYRIGHT: (C)2006,JPO&NCIPI</p> |
