Multiply-substituted protease variants with altered net charge for use in detergents

摘要

Methods of improving the performance of proteases in various detergent compositions are disclosed. The proteases are modified by substitution of amino acids to make the charge at those positions more negative or less positive compared to a precursor protease, or more positive or less negative compared to the precursor protease, with the result that the protease variant is more effective in a medium detergent concentration system than the precursor protease. Also provided are methods of improving performance of proteases in low, medium and high detergent concentration systems by substitution of amino acids to make the charge more positive or less negative compared to a precursor protease and by substitution of amino acids to make the charge more negative or less positive compared to the precursor protease.