摘要 |
A closed system for withdrawing, measuring, and isolating discrete quantities of liquid specimen for cryogenic preservation and recovery includes a fluoroplastic storage tube, an aspirating device, such as a syringe, and an impermeable barrier. The aspirating device can be used to meter exact amounts of specimen into the tube, and then to isolate the specimen within the confines of the tube during freezing, storage, thawing. The specimen in the tube can be withdrawn into the same syringe. While encapsulated, the specimen is protected from contact with air, gasses, and moisture in the cryogenic atmosphere. The specimen can be recovered from the frozen state without compromising sterility or exposure to any external environment.
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