| 摘要 |
Method of amplifying and detecting a nucleic acid target molecule contained in a test sample using a device having amplification primers and detectably labeled hybridization probes immobilized on the same surface of a solid support. Soluble amplicons produced in an in vitro nucleic acid amplification reaction employing the immobilized amplification primers are detected by the immobilized hybridization probes. Surface-immobilized amplification primers and hybridization probes are maintained in fluid communication throughout the procedure.
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