METHOD AND DEVICE FOR SEGMENTING A DIGITAL REPRESENTATION OF CELLS

摘要

The invention relates to a method and a device for segmenting a digital representation (22) of biological cells (2, 3), a method and a device for analysing the dynamic behaviour of biological cells (2, 3), and a method and a device for visualising the dynamic behaviour of biological cells (2, 3). The aim of the invention is to provide a segmenting method which has only low technical requirements, requires no special preparation or manipulation of the cells to be observed, and presupposes as few hypotheses as possible about cell characteristics. To this end, the inventive method comprises the following steps: a maximum gradient is determined (41) for each pixel of the representation as the maximum of the differences between an image value of the pixel and the corresponding image values of all or selected adjacent pixels; a segmenting threshold value is determined (42) on the basis of the frequencies of the maximum gradient; the pixels are classed (43) in an object category and an environment category by means of the segmenting threshold; and a segment region of the digital representation is formed (44) by means of a category merging method, especially a region growth method.