发明名称 METHOD FOR PRODUCTION OF DIAGNOSTICUM FOR DETERMINATION OF GENUS KLEBSIELLA BACTERIUM ENDOTOXIN
摘要 FIELD: medicine, veterinary and food processing industry. ^ SUBSTANCE: claimed method includes manufacturing of corn-shell-type latex, wherein corn represents polystyrene latex and shell represents styrene-zinc methacrylate copolymer latex in repeating unit mass ratio of 1:0.8-1:0.4. Then latex is sensitized with monoclonal antibodies of lgG2a and lgG2b fractions in 0.01 M glycine buffer at pH 7.2-8.8 without preliminary conditioning followed by incubation primarily at 37°C for 130-150 min and further at 8°C for 18 hours. Obtained diagnosticum has specificity of 97±0.6, sensibility up to 10 pg/ml and makes it possible to obtain investigation results for 10 min or less. ^ EFFECT: accelerated method for diagnosticum production. ^ 3 tbl
申请公布号 RU2266136(C2) 申请公布日期 2005.12.20
申请号 RU20030121794 申请日期 2003.07.17
申请人 发明人 BOKERIJA L.A.;NIJAZMATOV A.A.
分类号 A61K39/40;G01N33/547 主分类号 A61K39/40
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