| 摘要 |
FIELD: analytical methods in environmental protection and toxicology. ^ SUBSTANCE: subject of invention is drinking, natural, and waste water quality monitoring. Toxicity of aqueous medium is determined from variation in activity of animal brain plasma membrane Mg2+-ATPase activated by chlorine and/or bicarbonate ions. In one embodiment of invention, toxicity of aqueous medium is determined by mixing above plasma membranes with test aqueous medium adjusted to physiologic pH with phosphorus-free buffer followed by addition of solution containing Tris-ATP and magnesium ion source as well as chloride and/or bicarbonate ion(s) source, incubation until inorganic phosphorus is formed, and determination of toxicity from concentration of inorganic phosphorus. Brain plasma membranes used as indicator contain Mg2+-ATPase capable of being activated by chlorine and/or bicarbonate ions. ^ EFFECT: extended functional possibilities of method and use of reagent, increased sensitivity, and enabled determination of toxicity at lower concentration of various-type toxicants. ^ 20 cl, 6 tbl |
