METHOD OF PRODUCING LENS CELL AND LENS CELL OBTAINED BY THE METHOD

摘要

A method of producing a lens cell comprising the ES cell-maintaining step wherein ES cells are maintained by using a medium containing fibroblast growth factor FGF-2 at a concentration of from 2 to 50 ng/ml, and the differentiation-inducing step following the ES cell-maintaining step wherein the ES cells are transplanted and cultured in mouse skull bone marrow cells PA6 at a cell density of from 2 to 6.5 colonies/cm 2 to thereby induce the differentiation of the ES cells into lens cells. It is preferred to further employ the step of washing the undifferentiated ES cells once with the use of an ES differentiation medium between the above-described ES cell-maintaining step and the above- described differentiation-inducing step.