METHODS, COMPOSITIONS AND COMPOUND ASSAYS FOR INHIBITING AMYLOID-BETA PROTEIN PRODUCTION

摘要

A method for identifying compounds that inhibit amyloid-beta precursor protein processing in cells, comprising contacting a test compound with a HIT polypeptide, or fragment thereof, and measuring a compound-HIT property related to the production of amyloid-beta peptide. Cellular assays of the method measure indicators including cleaved protease substrates, phosphorylated kinase substrate, second messenger levels and/or amyloid beta peptide levels. Therapeutic methods, and pharmaceutical compositions including effective amyloid-beta precursor processing-inhibiting amounts of HIT expression inhibitors, are useful for treating conditions involving cognitive impairment such as Alzheimer's disease.