Apparatus with microtiter plate format for multiplexed arraying

摘要

The present invention relates generally to an apparatus and kit in the microtiter plate format for improved multiplexed microarraying. The apparatus overcomes current limitations with conventional polymeric and glass bottom microtiter plates. The invention also relates to an apparatus for conducting multiple biomolecular assays comprising a low self-fluorescent coated substrate having an upper and lower surface. The substrate upper surface has a pattern comprising a plurality of wells. A flexible superstructure having a plurality of through holes (i.e. openings) in a pattern that is similar to and aligns with the wells on the substrate is removably adherable to the upper surface of the substrate. Preferably, the superstructure is adhesively attachable to the substrate. A tray supports the substrate and the superstructure and acts as an alignment jig. In a preferred embodiment the substrate is low self-fluorescent glass and coated on the upper surface with a functional coating to which other biomolecules can bond.