METHOD FOR QUANTITATIVE SURFACE-ENHANCED RAMAN SPECTROSCOPY USING A CHEMICAL REFERENCE

摘要

A method for obtaining quantitative surface-enhanced Raman (SER) spectra that corrects for deficiencies of, and variations in, the materials and devices employed, especially the SER-active media utilized, employs a reference chemical, having an effective surface-enhanced Raman factor, of known concentration within the same SER experimental field of view as the analyte chemical being measured. Knowledge of the relative amounts of SER-scattering for the reference chemical and analyte chemical allows calculating the concentration of the latter to a high degree of accuracy and preci­sion.