| 摘要 |
<p>A light source (1, 23) that is ideal for use within a scanning microscope (10, 28) is described. The light source (1) comprises a laser source (2) coupled to a photonic crystal fibre (5) so as to generate a continuum spectrum from the fibre (5). Thereafter, selection apparatus, such as a bandpass filter (7) or a grating (24), is employed to select a wavelength range of the generated continuum spectrum. By matching the wavelength range to the peak absorption wavelengths of a particular fluorophore makes the light source ideal for use with the scanning microscope e.g. a confocal laser scanning microscope (10), a multiphoton laser scanning microscope or a fluorescence lifetime imaging microscope (28).</p> |
