METHOD FOR MEASURING POLYAMINES IN ERYTHROCYTES, DIAGNOSTIC METHOD AND KIT FOR MEASURING POLYAMINES IN ERYTHROCYTES

摘要

A method for measuring polyamines in erythrocytes in which a polyamine oxidase having a substrate specificity to spermine and spermidine or a polyamine oxidase having a substrate specificity to spermine only is used, an eluate in erythrocytes separated and purified from blood is reacted with these enzymes, and hydrogen peroxide formed is determined with a highly sensitive chromogen such as a diphenylamine-based chromogen. There is provided a method for measuring amounts of polyamines in erythrocytes easily with high precision. The method is effective for diagnosis of certain disease conditions or physiological conditions or the like of subjects.