BIOLOGICALLY ACTIVE ANTIBODIES RECOGNIZING A CELL SURFACE MOLECULE SELECTIVELY EXPRESSED ON LIVING MOUSE NATURAL TYPE ONE (I) INTERFERON PRODUCING CELLS

摘要

An isolated and characterized monoclonal antibody ("440c") prepared by immunizing Wistar/CRL rats subcutaneously with purified rat bone marrow IPC, fusing popliteal lymph nodes of the rats with SP2/0 myeloma spleen cells and differentially selecting for hybridome supernatants that successfully stain bone marrow derived IPC and fractions thereof using CDllc+ spleen cells and, differentially selecting for hybridoma supernatants from the CDllc+ spleen cells that successfully stain only CD 11 c+/Ly6c+/CD 11 b-/B220+ splenocytes to provide the monoclonal antibody.