HIGHLY SENSITIVE METHOD OF DETECTING SINGLE NUCLEOTIDE POLYMORPHISM WITH THE USE OF PHOTOCURRENT

摘要

It is intended to provide a novel SNP analysis method with the use of a photosensitizer and a double-stranded DNA. More specifically speaking, a novel SNP analysis method whereby an SNP nucleotide in a target DNA can be effectively and highly accurately detected while forming a double-strand via the hybridization of a probe DNA having the SNP site with the target DNA, i.e., an SNP typing method. Namely, a method of detecting a nucleotide at a single nucleotide polymorphism site in a target DNA which comprises hybridizing a probe DNA having the single nucleotide polymorphism site with a target DNA to form a double-stranded DNA complex and measuring a photocurrent generated by the double-stranded DNA complex.