PROTEIN EXPRESSION IN BACULOVIRUS VECTOR EXPRESSION SYSTEMS

摘要

The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a sufficient volume to contain at least 2 liters and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1 X 10 5 to 5 X 10 6 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E rns protein or fragments thereof in insect cell culture characterised by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 mug/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, alpha-units and/or beta-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 mu/ml.