发明名称 |
PROTEIN EXPRESSION IN BACULOVIRUS VECTOR EXPRESSION SYSTEMS |
摘要 |
The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a sufficient volume to contain at least 2 liters and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1 X 10 5 to 5 X 10 6 cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E rns protein or fragments thereof in insect cell culture characterised by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 mug/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, alpha-units and/or beta-units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 mu/ml.
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申请公布号 |
KR20050115341(A) |
申请公布日期 |
2005.12.07 |
申请号 |
KR20057022071 |
申请日期 |
2005.11.18 |
申请人 |
BAYER AKTIENGESELLSCHAFT;STICHTING INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID |
发明人 |
KRETZDORN DIETMAR;VAN DE WIEL DIRK FRANCISCUS MARINUS;DE SMIT ABRAHAM JOHANNES;MOORMANN ROBERTUS JACOBUS MARIA;HAMANN ERIK KEES |
分类号 |
C12N15/09;A61K39/00;A61K39/12;A61K39/187;A61P31/12;A61P31/14;C07K14/18;C07K14/185;C12N7/00;C12N15/40;C12N15/63;C12N15/866;C12P21/00;C12P21/02;(IPC1-7):C12N15/63 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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