ANTI-MICROBIAL PEPTIDE KNOWN AS HALOCYNTIN, GENE CODING FOR SAID PEPTIDE, VECTOR, TRANSFORMED ORGANISM AND COMPOSITION CONTAINING SAID PEPTIDE

摘要

An isolated peptide (I), designated halocyntin, and its derivatives and fragments, are new. Isolated peptide of formula (I), designated halocyntin, and its derivatives and fragments are new. Phe-Trp-Gly-His-Ile-Trp-Asn-Ala-Val-Lys-Arg-Val-Gly-Ala-Asn-Ala-Leu-His-Gly-Ala-Val-Thr-Gly-Ala-Leu-Ser (I) Independent claims are also included for the following: (1) isolated polypeptide (II) that includes (I); (2) isolated polynucleotide (III) that encodes (I) or (II); (3) cloning and/or expression vector that contains (III); (4) host organism that contains a vector of (3) or (III); (5) plant cell resistant to microbial disease that contains (III) or expresses (I) or (II); and (6) plant containing at least one cell of (5). ACTIVITY : Antibacterial; Fungicide; Plant Antibacterial; Plant Antifungal. The minimum bactericidal concentration of (I), in MicroM, was 1.56-3.13 against M. luteus; 6.25-12.5 against S. aureusor E. faecalis; 0.75-1.56 against B. megateriumand 3.13-6.25 against A. viridans. It was not appreciably active against Gram-negative species.. MECHANISM OF ACTION : Bacteriolysis.