Methods for refining concentrated enzyme solutions

摘要

Concentrated enzyme solutions are refined by a process comprising the steps of: (A) providing a concentrated enzyme solution comprised of insoluble solids; (B) separating the insoluble solids to produce a solids-free supernatant solution and (C) contacting the supernatant solution with a strongly basic anion exchanger carried out with a bed volume of from 1 to 10 to produce a decolorized protein solution. No precipitation takes place in the process. Instead, the target enzymes remain in solution throughout the entire process, for which purpose certain concentration ranges are particularly advantageous in regard to the yield to be obtained.