Microarray-based method for amplifying and detecting nucleic acids during a continuous process

摘要

The invention concerns methods, in the context of slide-based evaluation, for efficiency amplifying and detecting nucleic acids during a continuous process. The invention mainly concerns methods which consist in performing a polymerase chain reaction occurs, whereto a competitor element is added at the beginning of the reaction. The invention also concerns methods which consist in an hybridization in the presence of a molecule which, proximate to the hybridizing sequences, is bound to one of the hybridizing nucleic acid molecules