SYSTEM AND METHOD FOR SMOOTHING MELTING CURVE DATA

摘要

The present invention is a system and method for smoothing melting curve data to help identify a specific sequence of double stranded DNA sample after performing a polymerase chain reaction. The system comprises a plurality of measurements of a fluorescence and a temperature of a dye over a temperature range to create a raw array; calculating a collapse number using a temperature resolution that facilitates the smoothing of the melting curve data, a number of data points in the raw array, and a temperature data range from the raw array; calculating a plurality of fluorescence averages and a plurality of temperature averages from the collapse number and the raw array; and generating a smooth array from the plurality of fluorescence averages and the plurality of temperature averages wherein the smooth array decreases a variability in the raw array.