摘要 |
This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in a cell culture system comprising a cell culture medium and a cell attachment surface, under conditions wherein the acinar cells undergo a 3-4 fold expansion together with transdifferentiation into a modified cell phenotype (IP cells) showing characteristics of acinar cells and liver cells. The invention also relates to a method for transforming these IP cells to insulin-producing cells in vitro, comprising culturing the cells in a novel, defined medium. Also disclosed are suitable culture media for performing these methods, isolated cells having the phenotype of IP cells and/or produced by these methods, and kits for performing the methods. |
申请人 |
BECTON, DICKINSON AND COMPANY;NOVOCELL, INC.;PRESNELL, SHARON, C.;ROBBINS, NEIL;HEIDARAN, MOHAMMAD;HAALAND, PERRY;LATTA, PAUL, P.;SCHARP, DAVID, W.;COUTTS, MARGARET;MCINTYRE, CATHERINE |
发明人 |
PRESNELL, SHARON, C.;ROBBINS, NEIL;HEIDARAN, MOHAMMAD;HAALAND, PERRY;LATTA, PAUL, P.;SCHARP, DAVID, W.;COUTTS, MARGARET;MCINTYRE, CATHERINE |