PERFUSION SYSTEM AND APPARATUS FOR AUTOMATED MULTI-CHANNEL PATCH-CLAMP RECORDINGS UTILIZING INSIDE-OUT WHOLE-CELL CONFIGURATION

摘要

A system and method for high throughput patch clamp measurements to study th e effect of various chemicals on ion transfer channels is provided. One or mor e patch clamp configurations is established, each comprising a cell sealed to a pipette. The pipettes are affixed to a pipette fixture. The pipette fixture and a plate comprising one or more wells are relatively moved so that each cell is inside a well. Electrical properties of the cells are measured.