SUBSTRATES FOR HIGH-DENSITY CELL ARRAYS AND PREPARATION PROCESSES AND USES THEREOF

摘要

Culture substrates, cell arrays, automatic chemical dispensers and assay systems necessary for conveniently assaying multiple chemicals such as drugs and toxic agents are provided. Herein used is a substrate for a high-density cell array characterized in that it has a surface comprising an ordered array of discrete microdomains coated with a cell adhesive polymer and successively surrounded by a domain coated with a cell non-adhesive hydrophilic polymer and then a domain coated with a cell non-adhesive highly hydrophobic material. This substrate for a high-density cell array and an assay system using it can greatly reduce side effects of drugs and dramatically improve the performance of drug therapy for diseases by optimizing the type and concentration of the drug used (e.g., an anticancer agent). The present invention provides a very useful technology that can also be used for drug development, environmental impact assessment and basic life science researches.