发明名称 |
ELECTROFUSION CHAMBER AND METHOD FOR THE QUANTIFICATION OF HYBRIDOMA YIELDS |
摘要 |
The subject of the invention in the field of biomedical technology is the evaluation procedure for the determination of the yield of hybrid cells - hybridomas in a cell vaccine and cell products, which consists of measurements of the area of co-localized pixels of an image recorded by multichannel confocal microscopy and by using a reusable autoclavable electrofusion chamber to be used in the preparation of hybrid cells. The quantification of the yield of hybrid cells is based on measurements of the area of co-localized pixels and allows simultaneous visualization and documentation of the procedure. This approach has a wider applicability, for example also in cases in which a fraction of one or multiple cell types are present in a suspension or a structure. The subject of this invention is also a corresponding autoclavable electrofusion chamber, more precisely, a chamber for the subject of the invention in the field of biomedical technology is the evaluation procedure for the determination of the yield of hybrid cells - hybridomas in a cell vaccine and cell products, which consists of measurements of the area of co-localized pixels of an image recorded by multichannel confocal microscopy and by using a reusable autoclavable electrofusion chamber to be used in the preparation of hybrid cells. The quantification of the yield of hybrid cells is based on measurements of the area of co-localized pixels and allows simultaneous visualization and documentation of the procedure. This approach has a wider applicability, for example also in cases in which a fraction of one or multiple cell types are present in a suspension or a structure. The subject of this invention is also a corresponding autoclavable electrofusion chamber, more precisely, a chamber for fusion of biological cells in electric field. The invention brings a, new construction of a re-usable electrofusion chamber. |
申请公布号 |
WO2005063961(A3) |
申请公布日期 |
2005.11.24 |
申请号 |
WO2004SI00048 |
申请日期 |
2004.12.28 |
申请人 |
CELICA, D.O.O.;KREFT, MARKO;GRILC, SONJA;POBERAJ, IGOR;GABRIJEL, MATEJA;HAQUE CHOWDHURY, HELENA;ZOREC, ROBERT |
发明人 |
KREFT, MARKO;GRILC, SONJA;POBERAJ, IGOR;GABRIJEL, MATEJA;HAQUE CHOWDHURY, HELENA;ZOREC, ROBERT |
分类号 |
C12M1/34;C12M3/00;C12N5/12;C12N13/00 |
主分类号 |
C12M1/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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