发明名称 |
A METHOD FOR PURIFYING AND RECOVERING SILK PROTEINS USING MAGNETIC AFFINITY SEPARATION |
摘要 |
A method for the purification of recombinant silk proteins from a sample using magnetic affinity separation is described. The recombinant silk protein is expressed with an affinity tag which has a high binding affinity for an affinity ligand immobilized on magnetic particles. In the method, the processes of clarification of the crude silk protein extract, concentration of the product and purification of the product are combined in a single step involving the affinity capture of the spider silk protein onto the magnetic particles directly from the extract. The product yields are improved due to the reduced number of steps in the purification process. |
申请公布号 |
WO2005111068(A2) |
申请公布日期 |
2005.11.24 |
申请号 |
WO2005US14315 |
申请日期 |
2005.04.26 |
申请人 |
E.I. DUPONT DE NEMOURS AND COMPANY;HOFFMANN, CHRISTIAN;KELLER, KARSTEN |
发明人 |
HOFFMANN, CHRISTIAN;KELLER, KARSTEN |
分类号 |
C07K14/435;C12N5/06;C12P21/00;C12P21/06 |
主分类号 |
C07K14/435 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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