CHIMERIC MURINE MODEL COMPRISING HUMAN HEPATOCYTES USER FOR SCREENING COMPOUNDS

摘要

The invention relates to a method of making a chimeric murine model comprising (a) obtaining an immunocompromised murine host, which carries a transgene or a genetic defect responsible for murine host, which carries a transgene or a genetic defect responsible for murine hepatocyte death, (b) implanting human hepatocytes therein, (c) controlling non-adaptive defences and (d) recovering a chimeric murine model harbouring settled human hepatocytes capable of maintaining, differentiating and growing. The invention also discloses a chimeric murine model and relates to applications in pathogen studies having recourse to said model, including screening compounds or assessing efficacy of compounds in the treatment of pathogen infections or detrimental effects resulting from said infection. The invention also concerns the use of said model to evaluate the interest of compounds in treatment of patients, or in the study of metabolism of compounds contacted with said human hepatocytes.