Method and system for determining absolute mrna quantities

摘要

The present invention is particularly directed to a method and a system for determining absolute mRNA quantities, specifically on cDNA microarrays. The following steps and respective means are provided: providing a microarray; providing at least one or more dilution series of control spots on this microarray; hybridizing with a corresponding control cDNA of known concentration; deriving reference data from the hybridization and using said reference data to calculate absolute mRNA concentrations in one or more samples used.