摘要 |
Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed. |
申请人 |
HANDYLAB, INC.;WU, BETTY;ALTHAUS, JOHN, S.;BRAHMASANDRA, SUNDARESH, N.;HANDIQUE, KALYAN;PHADKE, NIKHIL |
发明人 |
WU, BETTY;ALTHAUS, JOHN, S.;BRAHMASANDRA, SUNDARESH, N.;HANDIQUE, KALYAN;PHADKE, NIKHIL |