发明名称 |
METHOD OF NMR MEASUREMENT OF PROTEIN |
摘要 |
<p>An object of the present invention is to provide a means for highly efficiently or quickly evaluating or determining the three-dimensional structure of a protein by conducting multidimensional multinuclear NMR measurement without performing a step of separating and purifying the synthesized labeled protein from other labeled proteins. The present invention provides a method for analyzing proteins, which comprises: synthesizing a protein of interest under conditions in which 90% or more of the synthesized labeled proteins constitute the protein of interest, when proteins are synthesized using a nucleic acid encoding the protein of interest as a template and also using substrate amino acids, a part or all of which have been labeled in such a way that NMR measurement is possible; and performing the NMR measurement using the above-described labeled proteins.</p> |
申请公布号 |
EP1596187(A1) |
申请公布日期 |
2005.11.16 |
申请号 |
EP20040709726 |
申请日期 |
2004.02.10 |
申请人 |
MITSUBISHI CHEMICAL CORPORATION;ENDOU, YAETE |
发明人 |
ENDOU, YAETA;KOHNO, TOSHIYUKI;MORITA, HAYATO |
分类号 |
G01N24/08;G01R33/465;(IPC1-7):G01N24/08 |
主分类号 |
G01N24/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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