METHOD OF CONSTRUCTING ANIMAL MODEL SUFFERING FROM LEFT VENTRICULAR DIASTOLIC DISORDER FOR EXAMINING HEART FAILURE AND METHOD OF EXAMINING REMEDY FOR HEART FAILURE CAUSED BY LEFT VENTRICULAR DIASTOLIC FAILURE WITH THE USE OF THE ANIMAL MODEL

摘要

It is intended to provide an animal model which enables the examination of the drug effect of a drug on left ventricular diastolic dysfunction such as heart failure caused by left ventricular diastolic failure. A method of constructing an animal model suffering from left ventricular diastolic disorder for examining a remedy for heart failure characterized by comprising injecting an aqueous calcium chloride solution into the vein of a small animal for laboratory use under anesthesia, and, while continuously injecting the aqueous calcium chloride solution into the vein, injecting an aqueous norepinephrine solution into the vein so as to elevate the left ventricular end-diastolic pressure of the animal's heart to a level higher than the normal left ventricular end-diastolic pressure; and a method of examining a remedy for heart failure caused by left ventricular diastolic failure by using an animal model suffering from left ventricular diastolic disorder for examining a remedy for heart failure characterized by comprising injecting an aqueous norepinephrine solution into the vein of an animal model for examining a remedy for heart failure which is constructed by the above method, measuring the left ventricular end-diastolic pressure, and comparing the left ventricular end-diastolic pressure thus measured with the normal left ventricular end-diastolic pressure before the injection of the aqueous norepinephrine solution.