DOUBLE-STRANDED RIBONUCLEIC ACID WITH INCREASED EFFECTIVENESS IN AN ORGANISM
摘要
The present invention relates to a method for the targeted selection of a double-stranded ribonucleic acid (dsRNA) consisting of two single strands that exhibits increased effectiveness in inhibiting the expression of a target gene by means of RNA interference, wherein at least end of the dsRNA comprises a nucleotide overhang of 1 to 4 unpaired nucleotides in length; wherein the unpaired nucleotide adjacent to the terminal nucleotide pair comprises a purine base; and wherein the terminal nucleotide pair on both ends of the dsRNA is a G-C pair, or at least two of the last four consecutive terminal nucleotide pairs are G-C pairs.
申请公布号
WO2005014782(B1)
申请公布日期
2005.11.10
申请号
WO2004US18848
申请日期
2004.06.14
申请人
ALNYLAM PHARMACEUTICALS;WOPPMANN, CLAUDIA;VORNLOCHER, HANS-PETER;HADWIGER, PHILIPP;JOHN, MATTHIAS;LIMMER, STEFAN
发明人
WOPPMANN, CLAUDIA;VORNLOCHER, HANS-PETER;HADWIGER, PHILIPP;JOHN, MATTHIAS;LIMMER, STEFAN