摘要 |
It is intended to provide novel primers for nucleic acid amplification to be used in detecting mRNA of a housekeeping gene, more particularly, confirming the amplification of beta -actin or GAPDH. More specifically speaking, primers containing oligonucleotides having nucleotide sequences represented by any of SEQ ID NOS: 2 and 4 to 49 (in the case of beta -actin) or oligonucleotides having nucleotide sequences represented by any of SEQ ID NOS: 52 to 96 (in the case of GAPDH) can be selected, combined and used. |