The present invention relates to a method for the isolation of proteins that comprise disulfide-bonds in their native conformation. Essentially, a method of the present makes the use of reducing agents such as &bgr;-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells.
申请公布号
EP1592703(A1)
申请公布日期
2005.11.09
申请号
EP20040706187
申请日期
2004.01.29
申请人
AKZO NOBEL N.V.
发明人
VAN DER MEIJDEN, PETER;VAN DEDEM, GIJSBERT, WILLEM, KAREL;EPPINK, MICHEL, HENDRIKUS, MARIA;WASSENAAR, ROELAND, WILHELMUS