发明名称 |
Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains |
摘要 |
A method of producing coryneform bacteria having improved amino acid or nucleic acid productivity comprising the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium to make it close to a consensus sequence, or introducing a change in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium by gene recombination to make it close to a consensus sequence, to obtain mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and selecting a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows the construction of a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield by recombination or mutation.
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申请公布号 |
US6962805(B2) |
申请公布日期 |
2005.11.08 |
申请号 |
US20000577005 |
申请日期 |
2000.05.25 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
ASAKURA YOKO;NAKAMURA JUN;KANNO SOHEI;SUGA MIKIKO;KIMURA EIICHIRO;ITO HISAO;MATSUI KAZUHIKO;OHSUMI TSUYOSHI;NAKAMATSU TSUYOSHI;KURAHASHI OSAMU |
分类号 |
C12N15/37;C12N1/21;C12N15/67;C12P13/04;C12P13/14;(IPC1-7):C12P13/14;C07H21/04;C12Q1/32;C12N15/74;C12N1/20 |
主分类号 |
C12N15/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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