发明名称 |
CHIMERIC GENE FORMED BY THE DNA SEQUENCES THAT ENCODE THE ANTIGENIC DETERMINANTS OF FOUR PROTEINS OF L. INFANTUM AND PROTEIN ENCODED BY SAID GENE, AND PHARMACEUTICAL COMPOSITION USEFUL FOR PREVENTING AND/OR TREATING LEISHMANIOSIS IN ANIMALS OR HUMANS |
摘要 |
Chimeric gene formed by the DNA sequences that encode the antigenic determinants of four proteins of L. infantum, useful for the serological diagnosis of canine Leishmaniosis and protein obtained, that consists of the prior employment of a cloning strategy. A clone is obtained which expresses the protein rLiPO-Ct-Q (pPQI). To this initial vector, by means of the use of suitable restriction targets, DNA fragments are sequentially added that are encoded in other proteins and after each cloning step the correct orientation of each one of the inserts reduces the size of the expression products, the complete nucleotide sequence of the final pPQV clone being determined. A chimeric polypeptide encoded by the chimeric gene is obtained with a molecular mass of 38 kD and with an isoelectric point of 7.37. This chimeric polypeptide is useful for preventing and/or treating Leishmaniosis in animals or humans.
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申请公布号 |
US6962704(B2) |
申请公布日期 |
2005.11.08 |
申请号 |
US20020237040 |
申请日期 |
2002.09.09 |
申请人 |
LABORATORIES LETI S.L. UNIPERSONAL |
发明人 |
BEDATE CARLOS ALONSO;REQUENA ROLANIA JOSE MARIA;SOTO ALVAREZ MANUEL |
分类号 |
A61K38/00;A61K39/00;C07K14/44;C12P21/04;(IPC1-7):A61K39/00;A61K39/002;A61K39/38 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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