发明名称 |
Novel method for the identification of clones conferring a desired biological property from an expression library |
摘要 |
The present invention relates to a novel method for the identification and/or characterization of clones conferring a desired biological property from an expression library. The method of the invention comprises the step of analyzing for the expression of at least one (poly)peptide, such as a tag expressed as a fusion protein, together with a recombinant insert of a clone of said expression library, wherein the clones of said expression library are arranged in arrayed form. Said (poly)peptide may be fused N-terminally or C-terminally to said insert. The method of the invention further comprises the steps of contacting a ligand specifically interacting with a (poly)peptide expressed by the insert of a clone conferring said desired biological property with a first replica of said library of clones in arrayed form and analyzing said library of clones for the occurrence of an interaction, and/or carrying out a hybridization or an oligonucleotide fingerprint with a nucleic acid probe specific for the insert of a clone conferring said desired biological property with a second replica of said library of clones arranged in arrayed form and analyzing said library of clones for the occurrence of a specific hybridization. Finally, the method of the invention requires the identification of clones wherein an expression of the at least one (poly)peptide in step (a) and/or an interaction in step (b) and/or a hybridization or an oligonucleotide fingerprint in step (c) can be detected. The present invention also relates to a kit useful for carrying out the method of the invention.
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申请公布号 |
US2005244854(A1) |
申请公布日期 |
2005.11.03 |
申请号 |
US20050045173 |
申请日期 |
2005.01.27 |
申请人 |
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN E.V. |
发明人 |
CAHILL DOLORES;BUSSOW KONRAD;WALTER GERALD;LEHRACH HANS;NIETFELD WILFRIED |
分类号 |
G01N33/50;C12N15/09;C12N15/10;C12Q1/68;C40B40/06;C40B60/14;G01N33/15;(IPC1-7):C12Q1/68;G01N33/53 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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