发明名称 |
Method of transplanting in a mammal and treating diabetes mellitus by administering a pseudo-islet like aggregate differentiated from a nestin-positive pancreatic stem cell |
摘要 |
Methods and compositions are described for the treatment of type I insulin-dependent diabetes mellitus and other conditions using newly identified stem cells that are capable of differentiation into a variety of pancreatic islet cells, including insulin-producing beta cells, as well as hepatocytes. Nestin has been identified as a molecular marker for pancreatic stem cells, while cytokeratin-19 serves as a marker for a distinct class of islet ductal cells. Methods are described whereby nestin-positive stem cells can be isolated from pancreatic islets and cultured to obtain further stem cells or pseudo-islet like structures. Methods for ex vivo differentiation of the pancreatic stem cells are disclosed. Methods are described whereby pancreatic stem cells can be isolated, expanded, and transplanted into a patient in need thereof, either allogeneically, isogeneically or xenogenically, to provide replacement for lost or damaged insulin-secreting cells or other cells.
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申请公布号 |
US2005244386(A1) |
申请公布日期 |
2005.11.03 |
申请号 |
US20040984645 |
申请日期 |
2004.11.09 |
申请人 |
THE GENERAL HOSPITAL CORPORATION |
发明人 |
HABENER JOEL E.;ZULEWSKI HENRYK;ABRAHAM ELIZABETH J.;VALLEJO MARIO;FAUSTMAN DENISE L.;THOMAS MELISSA K. |
分类号 |
C12N15/09;A01K67/027;A61K;A61K31/436;A61K31/7088;A61K35/12;A61K35/39;A61K38/00;A61K38/22;A61K39/395;A61K45/00;A61K48/00;A61P3/10;A61P37/06;A61P43/00;C12N;C12N5/02;C12N5/071;C12N5/074;C12Q1/00;G01N33/53;(IPC1-7):A61K45/00;C12N5/08 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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