MULTIDIMENSIONAL PROTEIN SEPARATION

摘要

In large scale proteome applications, protein separation is paramount to observing discrete changes and quantitative evaluation must coincide with qualitative protein identification for effective differential analysis. A four dimensional (4D) platform for resolving and differentially analyzing complex biological samples is presented. The system, collectively termed CAX-PAGE/RPLC-MSMS, combines bi-phasic ion-exchange chromatography (1<st> dimension) and polyacrylamide gel electrophoresis (2<nd> dimension) for protein separation, quantification and differential band targeting leading toward subsequent capillary reverse phase liquid chromatography (3<rd> dimension) and data dependant tandem mass spectrometry (4<th> dimension) for semi-quantitative and qualitative peptide analysis.