A protein having a dipeptide synthesizing activity or protein for dipeptide synthesis; a DNA coding for the protein having a dipeptide synthesizing activity or protein for dipeptide synthesis; a recombinant DNA containing the above DNA; a transformant wherein the recombinant DNA is incorporated; a process for producing the protein having a dipeptide synthesizing activity; an enzymic synthetic process for dipeptide using the protein having a dipeptide synthesizing activity or protein for dipeptide synthesis; and a process for producing a dipeptide with the use of, as an enzyme source, a culture of transformant or microbe capable of producing the protein having a dipeptide synthesizing activity or protein for dipeptide synthesis, etc.