Device for culturing cells in a vessel, useful e.g. for preparation of antibodies, comprising an oscillatory stirrer, membrane basket, containing carrier, and gasification membrane on the outside of the basket

摘要

Device (A) for cell culture in a vessel that has an oscillatory stirrer, fitted with a magnetic drive; a membrane basket that supports at least one microporous hollow fiber aeration membrane for the medium inside the vessel and, associated with the vessel, a closure plate. Device (A) for cell culture in a vessel that has an oscillatory stirrer, fitted with a magnetic drive; a membrane basket (containing a carrier) that supports at least one microporous hollow fiber aeration membrane for the medium inside the vessel and, associated with the vessel, a closure plate, having openings for inlet and outlet of gas, connected to the gas membrane by a flexible tube. The new feature is that carrier (20) of basket (2) includes, in addition to a gasification membrane (18), a microporous membrane-like coating (30) which, with a detachable base closure (24) and a covering element (19), forms a holder for receiving cell carrier material. (19) has a central aperture connected (by flexible line (6)), to a central aperture (9) in closure plate (3), so that basket (2), filled with cell carrier material, can be inoculated directly with the cells being grown.