发明名称 |
METHOD OF FUNCTIONALISING SOLID SUPPORTS |
摘要 |
<p>Functionalization of a solid support having hydroxy or hydride functions on its surface comprises attaching a bifunctional reagent carrying protected carboxy groups, then deprotection. Functionalization of a solid support having hydroxy or hydride functions on its surface comprises covalent fixation, through (hydro)silylation, of a compound (I), then deprotecting the carboxy groups that have not been deprotected during coupling. The new features are: (i) coupling is at 50 - 200 deg C using (I) in which A is monofunctional; and (ii) the deprotected carboxy groups, and any unreacted hydroxy/hydride groups that have not reacted with (I), are passivated by silanization. Independent claims are also included for the following: (1) functionalized supports prepared by the new method; (2) immobilizing biomolecules (A) on the supports of (1); and (3) supports prepared by (2). A-X-COOR (I) A = group that allows fixation to hydroxy or hydride functions; R = carboxy protecting group; X = 2 - 18C linear or branched hydrocarbyl chain, saturated or unsaturated and optionally interrupted by one or more of oxygen, sulfur and nitrogen.</p> |
申请公布号 |
EP1404631(B1) |
申请公布日期 |
2005.11.02 |
申请号 |
EP20020764946 |
申请日期 |
2002.07.05 |
申请人 |
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);ECOLE CENTRALE DE LYON |
发明人 |
DUGAS, VINCENT;CHEVALIER, YVES;SOUTEYRAND, ELIANE |
分类号 |
B01J31/02;B01J31/14;B01J31/16;C07B47/00;C07B61/00;C12N11/14;C12N15/09;C40B40/02;C40B50/06;G01N33/53;G01N33/543;G01N33/545;G01N33/552;(IPC1-7):C07B47/00;G01N33/547 |
主分类号 |
B01J31/02 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|