SIGNAL AMPLIFICATION METHOD FOR DETECTING EXPRESSED GENE

摘要

It is intended to provide a signal amplification method for detecting an expressed gene by which detection can be made at a low cost by a simple procedure within a short time without resort to any expensive enzymes and which enables detection corresponding to the original RNA length or expression amount because of using neither linear amplification nor PCR. Using a reverse transcription reaction and a self assembly reaction of forming a self-assembly due to the self assembly of oligonucleotide probes, the sensitivity of a DNA chip of detecting an expressed gene is elevated.