| 摘要 |
An isolated esterase gene coding for an esterase capable of causing asymmetric hydrolysis of an organic carboxylic acid ester of a cyclopentenolone of formula I: wherein R<SUB>1 </SUB>is hydrogen or methyl, and R<SUB>2 </SUB>is C<SUB>1</SUB>-C<SUB>10 </SUB>alkyl, C<SUB>2</SUB>-C<SUB>10 </SUB>alkenyl, C<SUB>2</SUB>-C<SUB>10 </SUB>alkynyl, C<SUB>1</SUB>-C<SUB>4 </SUB>haloalkyl, a C<SUB>5</SUB>-C<SUB>9 </SUB>aliphatic hydrocarbon moiety which may be optionally protected on the terminal hydroxyl group thereof, or a C<SUB>5</SUB>-C<SUB>9 </SUB>fatty acid residue which may be optionally protected on the terminal carboxyl group thereof, to produce the cyclopentenolone of formula I in (R)-form, and hybridizing to the base sequence of SEQ ID NO:1, is useful for the industrially favorable production of optically active cyclopentenolones of formula I which serve as the intermediates of drugs, agricultural chemicals or other active products.
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